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poly (di-dc)
Poly (Di Dc), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+(di-dc)/poly++di+dc+/pm39721498-100-31-33
Average 90 stars, based on 1 article reviews
Poly (Di Dc), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+(di-dc)/poly++di+dc+/pm39721498-100-31-33
Average 90 stars, based on 1 article reviews
poly (di-dc) - by Bioz Stars,
2026-10
90/100 stars
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Incubation:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Gel Shift:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Binding Assay:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Electrophoretic Mobility Shift Assay:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Modification:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Purification:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Recombinant:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. Electrophoresis:Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a. Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation. Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/ Gel-Shift binding buffer supplemented with Article Title: The Autoregulatory Feedback Loop of MicroRNA-21/Programmed Cell Death Protein 4/Activation Protein-1 (MiR-21/PDCD4/AP-1) as a Driving Force for Hepatic Fibrosis Development Article Snippet: For DNA binding reactions, 2 μl (∼16 μg) of nuclear extract was added to a 10-μl reaction (final) containing 50 n m biotin-labeled oligonucleotide and 5 μg of poly( Article Title: A conserved zinc finger transcription factor GATA involving in the hemocyte production of scallop Chlamys farreri. Article Snippet: GATA are a family of transcription factors characterized by their ability to bind to the DNA sequence “GATA”, and involved in a myriad of cellular processes.. GATA1/2/3 factors are known as the hematopoietic GATA factors, which play dominated roles in regulating hematopoiesis.. In the present study, a gene encoding GATA transcription factor (designed as CfGATA) was cloned and characterized from the scallop Chlamys farreri. Article Title: Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation Article Snippet: Nuclear extracts were prepared from VSMCs subjected to the indicated treatments and incubated with a biotin-labelled probe targeting the MMP2 promoter (5′-ggggaggagaggggcgggtcggacct-3′) in 5 × EMSA/Gel-Shift binding buffer supplemented with Article Title: Bacterial expression, refolding, functional characterization, and mass spectrometric identification of full-length human PPAR-gamma. Article Snippet: Binding reactions were carried out at room temperature for 30min using Article Title: CpxR promotes the carbapenem antibiotic resistance of Klebsiella pneumoniae by directly regulating the expression and the dissemination of bla KPC on the IncFII conjugative plasmid. Article Snippet: Electrophoretic mobility shift assays Electrophoretic mobility shift assays (EMSAs) were carried out by mixing wild-type or mutated CpxR at a range of concentrations with EMSA/Gel-Shift Binding Buffer (poly(dI-dC), DTT, Glycerol, EDTA, NaCl, MgCl2, Article Title: The essential roles of core binding factors CfRunt and CfCBFβ in hemocyte production of scallop Chlamys farreri. Article Snippet: Core binding factor (CBF) is a family of heterodimeric transcription factors composed of a DNA-binding CBFa subunit and a non-DNA-binding CBFb subunit, which plays critical roles in regulating hematopoiesis, osteogenesis and neurogenesis.. In the present study, two genes encoding Runt (designed as CfRunt) and CBFb (designed as CfCBFb) were cloned and characterized from scallop Chlamys farreri.. The fulllength cDNA of CfRunt and CfCBFb consists of 2128 bp and 1729 bp encoding a predicted polypeptide of 530 and 183 amino acids with a conserved Runt domain and CBFb domain, respectively. |